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Apr 22, 2024
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Abstract
Introduction: Pulp and periapical tissue problems are 7th out of the top 10 diseases. Propolis from stingless bees is a natural substance that has been proven its efficacy in treating the diseases. Additionally, it is categorized safe for humans. This study aimed to determine the antibacterial activity of stingless bee propolis (Heterotrigona itama) ethanol extract on dental root canal bacterial growth in vitro. Method: This study was an experimental study with a posttest-only control group design and consisting of six treatment groups with concentrations of 3,000; 6,000; 12,000; and 24,000 ppm of stingless bee propolis, as well as a positive and negative control. Each treatment was repeated four times. Result: The result showed that there were no clear zones at concentrations of 24,000 ppm, but a clear zones appeared at concentrations of 3,000; 6,000; and 12,000 ppm. Stingless bee propolis ethanol extract is efficient against bacteria. At a concentration of 3,000 ppm, the inhibition zone's maximum circumference was visible.
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This work is licensed under a Creative Commons Attribution 4.0 International License.
How to Cite
Takdir, A. Q., Purnamasari , C. B., Ompusunggu , N. Y., Paramita, S., & Yani, S. (2024). Antibacterial Activity of Stingless Bee Propolis (Heterotrigona itama) Ethanol Extract on Dental Root Canal Bacteria Growth in Vitro. International Journal of Multidisciplinary: Applied Business and Education Research, 5(4), 1127-1133. https://doi.org/10.11594/ijmaber.05.04.01
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Febrianifa E, Hadriyanto W, Kristanti Y (2016) Perbedaan daya antibakteri siler saluran akar berbahan dasar seng oksid eugenol, resin epoksi dan mineral trioxide aggre-gate terhadap Enterococcus faecalis. Jurnal Kedokteran Gigi 7(2): 41-47.
Garg N, Garg A (2010) Textbook of Endodon-tics. 2nd Ed. New Delhi, Jaypee Brothers Medical Publishers
Goetie IH, Sundu R, Supriningrum R (2022) Uji aktivitas antibakteri ekstrak kulit batang sekilang (Embelia borneensis Scheff) ter-hadap Bakteri Escherichia coli dan Staphylococcus aureus menggunakan metode disc diffusion. Jurnal Riset Kefarmasian Indonesia 4(2): 144-155.
Gopikrishna V (2021) Grossman's Endodontic Practice. 14th Edition. India, Wolters Kluwer Health
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Lim JR, Chua LS, Soo J. Study of stingless bee (Heterotrigona itama) propolis using LC-MS/MS and TGA-FTIR. Applied Food Re-search 2023; 3: 100252.
Lutpiatina L (2015) Efektivitas ekstrak propo-lis lebah kelulut (Trigona spp) dalam menghambat pertumbuhan Salmonella typhi, Staphylococcus aureus dan Can-dida albicans. Jurnal Skala Kesehatan 6(1).
Mubarak Z, Chismirina S, Daulay HH (2016) Aktivitas antibakteri ekstrak propolis alami dari sarang lebah terhadap per-tumbuhan Enterococcus faecalis. Journal of Syiah Kuala Dentistry Society 1(2): 175-186.
Mulyawati E (2011) Peran bahan disinfeksi pada perawatan saluran akar. Majalah Kedokteran Gigi 18(2): 205-209.
Nomer NMGR, Duniaji AS, Nocianitri KA (2019) Kandungan senyawa flavonoid dan antosianin ekstrak kayu secang (Caesalpinia sappan L.) serta aktivitas an-tibakteri terhadap Vibrio cholerae. Jurnal Ilmu dan Teknologi Pangan 8(2): 216-225.
Novitasari M, Nugroho R (2017) Frekuensi kegagalan pengisian saluran akar dengan teknik preparasi step back pada gigi be-rakar ganda di Rumah Sakit Gigi dan Mulut Universitas Jember 2011-2016. Pustaka Kesehatan 5(2): 331-338.
Pribadi A (2020) Produktivitas panen propolis mentah lebah Trigona itama Cockerell (Hymenoptera: Apidae) menggunakan propolis trap dan manipulasi lingkungan di Riau. Majalah Ilmiah Biologi BI-OSFERA: A Scientific Journal 37(2): 60-68.
Ristianti N, Marsono M (2015) Perbedaan efektifitas obat kumur herbal dan non herbal terhadap akumulasi plak di dalam rongga mulut. Medali Jurnal: Media Den-tal Intelektual 2(1): 31-36
Saroinsong MS, Kandou FE, Papu A, Singkoh MF (2014) Uji daya hambat ekstrak metanol beberapa jenis porifera terhadap Bakteri Escherichia coli dan Staphylococ-cus aureus. Jurnal MIPA 3(2): 129-133.
Selvan AK, Prabhu T (2010) Extraction of propolis from beehives and characteriza-tion of its constituens and medicinal properties: A review. International Jour-nal of Advanced Engineering Technology. 1(3): 50-53.
Septiani S, Dewi EN, Wijayanti I (2017) Aktivi-tas antibakteri ekstrak lamun (Cymo-docea rotundata) terhadap Bakteri Staphylococcus aureus dan Escherichia coli. Saintek Perikanan: Indonesian Jour-nal of Fisheries Science and Technology 13(1): 1-6.
Silviana S, Asri MT (2022) Aktivitas antibakteri ekstrak etanol lichen usnea sp. terhadap pertumbuhan bakteri Ralstonia sola-nacearum. Sains dan Matematika 7(1): 20-25.
Starr CK (2021) Encyclopedia of Social Insects. 1st Ed. Springer International Publishing.
Šuran J, Cepanec I, Mašek T, Radić B, Radić S, Tlak Gajger I, Vlainić J (2021) Propolis extract and its bioactive compounds—From traditional to modern extraction technologies. Molecules. 26(10): 2930.
Tanumihardja M (2010) Larutan irigasi salu-ran akar. Journal of Dentomaxillofacial Science 9(2): 108-115.
Thakur V, Kaur M, Jamwal P, Thakur B (2020) 2% Chlorhexidine in root canal treat-ment: A review. Journal of Current Medi-cal Research and Opinion 3(12): 770-774.
Wahjuningrum DA, Subijanto A (2014) The antibiofilm activity of extract propolis against biofilm Enterococcus faecalis as herbal medicine potential in root canal treatment. ESP Endodontic Society of the Philippines 8(1): 15-18.
Wardaniati I, Gusmawarni V (2021) Uji aktivi-tas antibakteri ekstrak etanol propolis terhadap Streptococcus mutans. Jurnal Farmasi Higea 13(2): 115-123.
Yoga IGKM, Giri PRK, Suarjana K (2018) Gam-baran kejadian pulpitis di wilayah kerja Puskesmas Dawan I Klungkung. Bali Den-tal Journal 2(2): 95-99.
Yuwono B (2015) Penatalaksanaan pencabu-tan gigi dengan kondisi sisa akar (Gan-gren Radik). STOMATOGNATIC-Jurnal Kedokteran Gigi 7(2): 89-95.
Zeniusa P, Ramadhian MR, Nasution SH, Kari-ma N (2019) Uji daya hambat ekstrak etanol teh hijau terhadap Escherichia coli secara in vitro. Majority 8(2): 136-143.
Zulfa AF, Batistuta MA, Kustiawan PM (2022) Aktivitas antibakteri fraksi etil asetat dari propolis lebah kelulut Geniotrigona tho-racica terhadap Bakteri Staphyloccocus aureus. Lumbung Farmasi: Jurnal Ilmu Kefarmasian 3(2): 215-220.
Ariyani H, Nazemi M, Hamidah H, Kurniati M (2018) Uji efektivitas antibakteri ekstrak kulit limau kuit (Cytrus hystrix DC) ter-hadap Beberapa Bakteri. JCPS (Journal of Current Pharmaceutical Sciences) 2(1): 136-141.
Arung ET, Dikarulin SA, Listyaningrum DAD, Ananda BS, Putri TA, Amirta R, … Rama-dhan R (2022) Uji Aktivitas Antibakteri Ekstrak Propolis Lebah Heterotrigona itama dari Beberapa Lokasi Budidaya di Kalimantan Timur terhadap Bakteri Pro-pionibacterium acnes. ULIN: Jurnal Hutan Tropis. 6(2): 121-125.
Bidjuni M, Harapan IK (2019) Penyakit pulpa pada pasien pengunjung poliklinik gigi di Rumah Sakit Umum Daerah Kota Ko-tamobagu Tahun 2016-2018. JIGIM (Jurnal Ilmiah Gigi dan Mulut) 2(2): 83-88.
Febrianifa E, Hadriyanto W, Kristanti Y (2016) Perbedaan daya antibakteri siler saluran akar berbahan dasar seng oksid eugenol, resin epoksi dan mineral trioxide aggre-gate terhadap Enterococcus faecalis. Jurnal Kedokteran Gigi 7(2): 41-47.
Garg N, Garg A (2010) Textbook of Endodon-tics. 2nd Ed. New Delhi, Jaypee Brothers Medical Publishers
Goetie IH, Sundu R, Supriningrum R (2022) Uji aktivitas antibakteri ekstrak kulit batang sekilang (Embelia borneensis Scheff) ter-hadap Bakteri Escherichia coli dan Staphylococcus aureus menggunakan metode disc diffusion. Jurnal Riset Kefarmasian Indonesia 4(2): 144-155.
Gopikrishna V (2021) Grossman's Endodontic Practice. 14th Edition. India, Wolters Kluwer Health
Indriana RA, Astuti P, Kurniawati A (2017) Uji daya hambat ekstrak metanol daun ungu (Graptophillum pictum (L.) Griff) ter-hadap pertumbuhan bakteri saluran akar gigi. Pustaka Kesehatan 5(1): 145-150.
Lim JR, Chua LS, Soo J. Study of stingless bee (Heterotrigona itama) propolis using LC-MS/MS and TGA-FTIR. Applied Food Re-search 2023; 3: 100252.
Lutpiatina L (2015) Efektivitas ekstrak propo-lis lebah kelulut (Trigona spp) dalam menghambat pertumbuhan Salmonella typhi, Staphylococcus aureus dan Can-dida albicans. Jurnal Skala Kesehatan 6(1).
Mubarak Z, Chismirina S, Daulay HH (2016) Aktivitas antibakteri ekstrak propolis alami dari sarang lebah terhadap per-tumbuhan Enterococcus faecalis. Journal of Syiah Kuala Dentistry Society 1(2): 175-186.
Mulyawati E (2011) Peran bahan disinfeksi pada perawatan saluran akar. Majalah Kedokteran Gigi 18(2): 205-209.
Nomer NMGR, Duniaji AS, Nocianitri KA (2019) Kandungan senyawa flavonoid dan antosianin ekstrak kayu secang (Caesalpinia sappan L.) serta aktivitas an-tibakteri terhadap Vibrio cholerae. Jurnal Ilmu dan Teknologi Pangan 8(2): 216-225.
Novitasari M, Nugroho R (2017) Frekuensi kegagalan pengisian saluran akar dengan teknik preparasi step back pada gigi be-rakar ganda di Rumah Sakit Gigi dan Mulut Universitas Jember 2011-2016. Pustaka Kesehatan 5(2): 331-338.
Pribadi A (2020) Produktivitas panen propolis mentah lebah Trigona itama Cockerell (Hymenoptera: Apidae) menggunakan propolis trap dan manipulasi lingkungan di Riau. Majalah Ilmiah Biologi BI-OSFERA: A Scientific Journal 37(2): 60-68.
Ristianti N, Marsono M (2015) Perbedaan efektifitas obat kumur herbal dan non herbal terhadap akumulasi plak di dalam rongga mulut. Medali Jurnal: Media Den-tal Intelektual 2(1): 31-36
Saroinsong MS, Kandou FE, Papu A, Singkoh MF (2014) Uji daya hambat ekstrak metanol beberapa jenis porifera terhadap Bakteri Escherichia coli dan Staphylococ-cus aureus. Jurnal MIPA 3(2): 129-133.
Selvan AK, Prabhu T (2010) Extraction of propolis from beehives and characteriza-tion of its constituens and medicinal properties: A review. International Jour-nal of Advanced Engineering Technology. 1(3): 50-53.
Septiani S, Dewi EN, Wijayanti I (2017) Aktivi-tas antibakteri ekstrak lamun (Cymo-docea rotundata) terhadap Bakteri Staphylococcus aureus dan Escherichia coli. Saintek Perikanan: Indonesian Jour-nal of Fisheries Science and Technology 13(1): 1-6.
Silviana S, Asri MT (2022) Aktivitas antibakteri ekstrak etanol lichen usnea sp. terhadap pertumbuhan bakteri Ralstonia sola-nacearum. Sains dan Matematika 7(1): 20-25.
Starr CK (2021) Encyclopedia of Social Insects. 1st Ed. Springer International Publishing.
Šuran J, Cepanec I, Mašek T, Radić B, Radić S, Tlak Gajger I, Vlainić J (2021) Propolis extract and its bioactive compounds—From traditional to modern extraction technologies. Molecules. 26(10): 2930.
Tanumihardja M (2010) Larutan irigasi salu-ran akar. Journal of Dentomaxillofacial Science 9(2): 108-115.
Thakur V, Kaur M, Jamwal P, Thakur B (2020) 2% Chlorhexidine in root canal treat-ment: A review. Journal of Current Medi-cal Research and Opinion 3(12): 770-774.
Wahjuningrum DA, Subijanto A (2014) The antibiofilm activity of extract propolis against biofilm Enterococcus faecalis as herbal medicine potential in root canal treatment. ESP Endodontic Society of the Philippines 8(1): 15-18.
Wardaniati I, Gusmawarni V (2021) Uji aktivi-tas antibakteri ekstrak etanol propolis terhadap Streptococcus mutans. Jurnal Farmasi Higea 13(2): 115-123.
Yoga IGKM, Giri PRK, Suarjana K (2018) Gam-baran kejadian pulpitis di wilayah kerja Puskesmas Dawan I Klungkung. Bali Den-tal Journal 2(2): 95-99.
Yuwono B (2015) Penatalaksanaan pencabu-tan gigi dengan kondisi sisa akar (Gan-gren Radik). STOMATOGNATIC-Jurnal Kedokteran Gigi 7(2): 89-95.
Zeniusa P, Ramadhian MR, Nasution SH, Kari-ma N (2019) Uji daya hambat ekstrak etanol teh hijau terhadap Escherichia coli secara in vitro. Majority 8(2): 136-143.
Zulfa AF, Batistuta MA, Kustiawan PM (2022) Aktivitas antibakteri fraksi etil asetat dari propolis lebah kelulut Geniotrigona tho-racica terhadap Bakteri Staphyloccocus aureus. Lumbung Farmasi: Jurnal Ilmu Kefarmasian 3(2): 215-220.