Main Article Content
Abstract
This study aims to assess parasitic contamination in lettuce (Lactuca sativa) from selected public markets in Davao City, Philippines. Using a quantitative cross-sectional design, a total of 60 samples were collected from Agdao, Bankerohan, and Toril Public Markets. Each sample was placed in sterile plastic bags and sent to the University of Mindanao Medical Technology Laboratory for examination. Using the method of Hoffman, Pons, and Janer, the samples were washed with saline, filtered, and examined using direct wet mount technique after overnight sedimentation. Four samples were positive for parasites, corresponding to a total prevalence of 6.67 percent. The parasites identified were Strongyloides stercoralis, Balantidium spp., filariform larva, and a trematode egg. It was also found that there is no significant difference in parasitic contamination among the three public markets. The presence of parasites in commonly consumed raw vegetables like lettuce poses a significant threat to consumer safety. Hence, the researchers recommend that local health agencies in the community strengthen educational initiatives targeting vendors, food handlers, consumers, and the general public on proper vegetable handling and safe preparation practices. It is recommended that further research be conducted on effective methods for washing lettuce and other vegetables before consumption to safeguard the public from possible parasitic infections and outbreaks in the future.
Article Details
This work is licensed under a Creative Commons Attribution 4.0 International License.
References
Ahmed, A., Sayed, F., Galal, L., Ismail, T., & Gaber, M. (2020). Detection of parasites contaminating raw consumable vegeta-bles in Assiut City, Assiut Governorate, Egypt. Journal of the Egyptian Society of Parasitology, 50(3), 557–564. https://doi.org/10.21608/jesp.2020.131086
Aiwny Cássia, J. A., Anelise Andrade, d. S., & Marco Antônio Andrade, d. S. (2020). Prevalence of enteroparasites in vegeta-bles marketed in the city of Jaguaré, Es-pírito Santo, Brazil. Revista de Salud Pú-blica, 22(4), 1–6. https://doi.org/10.15446/rsap.v22n4.77097
Al Nahhas, S., & Aboualchamat, G. (2020). In-vestigation of parasitic contamination of salad vegetables sold by street vendors in city markets in Damascus, Syria. Food and Waterborne Parasitology, 21, e00090. https://doi.org/10.1016/j.fawpar.2020.e00090
Alemu, G., Nega, M., & Alemu, M. (2020). Para-sitic contamination of fruits and vegeta-bles collected from local markets of Bahir Dar City, Northwest Ethiopia. Research and Reports in Tropical Medicine, 11, 17–25. https://doi.org/10.2147/RRTM.S244737
Alshareef, S. A., Abdulsalam, A., Alghanaei, R. A., Salim, E., & Chibani, M. (2019). Para-sitic contamination of raw vegetables sampled from different farm locations in Brack Al-Shati, Libya. Journal of Pure & Applied Sciences, 18(4). https://doi.org/10.51984/jopas.v18i4.439
Anand, S. S., Hawkes, C., de Souza, R. J., et al. (2015). Food consumption and its impact on cardiovascular disease: Importance of solutions focused on the globalized food system: A report from the workshop con-vened by the World Heart Federation. Journal of the American College of Cardi-ology, 66(14), 1590–1614. https://doi.org/10.1016/j.jacc.2015.07.050
Bekele, F., Tefera, T., Biresaw, G., & Yohannes, T. (2017). Parasitic contamination of raw vegetables and fruits collected from se-lected local markets in Arba Minch town, Southern Ethiopia. Infectious Diseases of Poverty, 6(1), 19. https://doi.org/10.1186/s40249-016-0226-6
Belizario, V. Y., Jr., Totañes, F. I., de Leon, W. U., Lumampao, Y. F., & Ciro, R. N. (2011). Soil-transmitted helminth and other in-testinal parasitic infections among school children in indigenous people communi-ties in Davao del Norte, Philippines. Acta Tropica, 120(Suppl 1), S12–S18. https://doi.org/10.1016/j.actatropica.2011.02.010
Berrouch, S., Escotte-Binet, S., Amraouza, Y., et al. (2020). Cryptosporidium spp., Giardia duodenalis and Toxoplasma gondii detec-tion in fresh vegetables consumed in Marrakech, Morocco. African Health Sci-ences, 20(4), 1669–1678. https://doi.org/10.4314/ahs.v20i4.19
Brooker, S. J., Ziumbe, K., Negussu, N., Crow-ley, S., & Hammami, M. (2021). Neglected tropical disease control in a world with COVID-19: An opportunity and a necessi-ty for innovation. Transactions of the Royal Society of Tropical Medicine and Hygiene, 115(3), 205–207. https://doi.org/10.1093/trstmh/traa157
Célio, A., Guido, N., Izaidino, M., Adérito, N., Yazido, M., & Jorge, M. (2021). Parasito-logical assessment of lettuce quality in the Maxixe City markets, Mozambique. Journal of Bacteriology & Parasitology, S8, 002. https://doi.org/10.35248/2155-9597.21.s8.002
de Carvalho Souza Machado, T., Cristina Apo-linario Borges, C., Coelho Ribeiro Men-donca, F., & Cristina Euzebio Pereira Dias de Oliveira, B. (2020). Parasitological evaluation of lettuce served in school meals at a federal state school in Rio de Janeiro, Brazil. Revista de Patologia Trop-ical, 49(1), 33–44. https://doi.org/10.5216/rpt.v49i1.61879
El Bakri, A., Hussein, N. M., Ibrahim, Z. A., Ha-san, H., & AbuOdeh, R. (2020). Intestinal parasite detection in assorted vegetables in the United Arab Emirates. Oman Medi-cal Journal, 35(3), e128. https://doi.org/10.5001/omj.2020.46
Hajare, S. T., Gobena, R. K., Chauhan, N. M., & Erniso, F. (2021). Prevalence of intestinal parasite infections and their associated factors among food handlers working in selected catering establishments from Bule Hora, Ethiopia. BioMed Research In-ternational, 2021, 6669742. https://doi.org/10.1155/2021/6669742
Isazadeh, M., Mirzaii-Dizgah, I., Shaddel, M., & Homayouni, M. M. (2020). The preva-lence of parasitic contamination of fresh vegetables in Tehran, Iran. Turkish Jour-nal of Parasitology, 44(3), 143–148. https://doi.org/10.4274/tpd.galenos.2020.6469
Kassaw, M. W., Abebe, A. M., Tlaye, K. G., Zemariam, A. B., & Abate, B. B. (2019). Prevalence and risk factors of intestinal parasitic infestations among preschool children in Sekota town, Waghimra zone, Ethiopia. BMC Pediatrics, 19(1), 437. https://doi.org/10.1186/s12887-019-1774-2
Kudah, C., Sovoe, S., & Baiden, F. (2018). Para-sitic contamination of commonly con-sumed vegetables in two markets in Gha-na. Ghana Medical Journal, 52(2), 88–93. https://doi.org/10.4314/gmj.v52i2.5
Leonardo, L., Hernandez, L., Magturo, T. C., et al. (2020). Current status of neglected tropical diseases (NTDs) in the Philip-pines. Acta Tropica, 203, 105284. https://doi.org/10.1016/j.actatropica.2019.105284
Li, J., Wang, Z., Karim, R. M., & Zhang, L. (2020). Detection of human intestinal protozoan parasites in vegetables and fruits: A review. Parasites & Vectors, 13, 380. https://doi.org/10.1186/s13071-020-04255-3
Mirzaei, Y., Mohammadi, C., Ahmad, S. F., Hamad, P. M., & Samiei, A. (2021). Preva-lence of intestinal parasites in raw vege-tables consumed in Soran city, Kurdistan Region, Iraq. Annals of Parasitology, 67(2), 275–279. https://doi.org/10.17420/ap6702.339
Monterde, V., & Ekman, J. (2021). Food han-dling practices for fresh-cut vegetables at wet markets and supermarkets in Davao City, Philippines. BANWA Series B. http://ojs.upmin.edu.ph/index.php/banwa-b/article/view/493
Muchanga, I., Alfredo, C., Jorge, M., Yazido, M. M., & Nchowela, G. N. (2021). Parasito-logical assessment of lettuce quality in the Maxixe City markets, Mozambique. [Unpublished Manuscript].
Obebe, O. O., Aluko, O. O., Falohun, O. O., Akinlabi, K. B., & Onyiche, T. E. (2020). Parasitic contamination and public health risk of commonly consumed vegetables in Ibadan-Nigeria. Pan African Medical Journal, 36, 126. https://doi.org/10.11604/pamj.2020.36.126.19364
Ordoñez, K. N., Lim, Y. A. L., Goh, X. T., & Paller, V. G. V. (2021). Parasite contami-nation of freshly harvested vegetables from selected organic and conventional farms in the Philippines. [Journal article]. https://www.ukdr.uplb.edu.ph/journal-articles/977
Paller, V. G. V., Macalinao-Ramirez, C. A., & Bandal, M. Z. (2022). Environmental con-tamination with parasites in selected ru-ral farms in the Philippines: Impacts of farming practices on leafy greens food safety. Parasitology, 149(4), 482–489. https://doi.org/10.1017/S0031182021002094
Punsawad, C., Phasuk, N., Thongtup, K., et al. (2019). Prevalence of parasitic contami-nation of raw vegetables in Nakhon Si Thammarat province, southern Thailand. BMC Public Health, 19, 34. https://doi.org/10.1186/s12889-018-6358-9
Rodrigues, A. C., Silva, M. D., Pereira, R. Â., & Pinto, L. C. (2020). Prevalence of contam-ination by intestinal parasites in vegeta-bles (Lactuca sativa L. and Coriandrum sativum L.) sold in markets in Belém, northern Brazil. Journal of the Science of Food and Agriculture, 100(7), 2859–2865. https://doi.org/10.1002/jsfa.10265
Ross, A. G., Papier, K., Luceres-Catubig, R., Chau, T. N., Inobaya, M. T., & Ng, S. K. (2017). Poverty, dietary intake, intestinal parasites, and nutritional status among school-age children in the rural Philip-pines. Tropical Medicine and Infectious Disease, 2(4), 49. https://doi.org/10.3390/tropicalmed2040049
Sebastian, R. S., Wilkinson Enns, C., Goldman, J. D., Hoy, M. K., & Moshfegh, A. J. (2019). Findings from What We Eat in America, National Health and Nutrition Examina-tion Survey 2011–2014 support salad consumption as an effective strategy for improving adherence to dietary recom-mendations. Public Health Nutrition, 22(6), 976–987. https://doi.org/10.1017/S1368980018003695
Vazhavandal, G., Ajitha, S., Uma, A., & Prabhu-saran, N. (2020). Study on parasitic con-tamination of common edible fruits and vegetables sold in local markets of Tiru-chirappalli, South India. Indian Journal of Microbiology Research, 7(4), 362–368. https://doi.org/10.18231/j.ijmr.2020.065
Vizon, K. C. C., Battad, Z. G., & Castillo, D. S. C. (2019). Contamination of food-borne parasites from green-leafy vegetables sold in public markets of San Jose City, Nueva Ecija, Philippines. Journal of Para-sitic Diseases, 43(4), 651–657. https://doi.org/10.1007/s12639-019-01144-0